季维克，华中科技大学同济基础医学院教授，国家优青。

weike_ji1985@163.com

研究方向

真核细胞的生命过程依赖于细胞内功能特化的细胞器。细胞器的调控失衡与重大的疾病（神经退行性疾病、癌症、糖尿病等）直接相关。细胞器的组成和结构处于高度动态变化之中，不同细胞器之间通过物理接触，形成膜接触位点，进行物质交换和信号传递，构成胞内膜功能网络，执行复杂的生物学功能。我们通过siRNA 基因组文库筛选和质谱鉴定，筛选细胞器膜接触位点形成和调控的关键新因子，利用超高分辨率成像、电镜断层重构、体外生化重构等细胞生物学前沿技术，解析这些关键因子的作用机制，最终为相关重大疾病的诊疗策略提供科学依据和潜在治疗线索。

研究成果

近五年，申请人在细胞器动态调控与相互作用领域开展研究，取得一定进展。相关研究工作发表在Journal of Cell Biology (2023; 唯一通讯作者)，Cell Death and Differentiation (2022，共同通讯作者，第一完成单位)，Nature Communications (2021， 唯一通讯作者)，Journal of Cell Science (2021; 唯一通讯作者)，Molecular Biology of the Cell (2021， 唯一通讯作者) 及 Journal of Cell Biology (2017; 唯一第一作者)等国际权威期刊。

主持科研项目

国家自然科学基金优秀青年项目(32122025) 2022 – 2024

国家自然科学基金重大研究计划(91854109) 2019 – 2024

国家自然科学青年基金项目(31701170) 2018 – 2020

湖北省自然科学基金面上项目2018 – 2019

因研究需要，本研究组拟招收多名硕士/博士/博后。研究组轻松活跃，科研氛围浓郁。研究组依托华中科技大学和深圳湾实验室，具备充足的研究资源，欢迎对细胞生物学以及重大疾病致病机理研究感兴趣的同学加入我们！

研究论文

1. Yuanjiao Du(#), Weiping Chang, Lei Gao, Lin Deng, and Wei-Ke Ji* (2023) Tex2 is required for lysosomal

functions at TMEM55-dependent ER contacts. Journal of Cell Biology. Vol. 222 No. 4 e202205133.

2. Qingzhu Chu(#), Jingru Wang(#), Yuanjiao Du, Tiantian Zhou, Anbing Shi, Juan Xiong*, Wei-Ke Ji* and Lin Deng* (2022) Oligomeric CHMP7 mediates three-way ER junctions and ER-mitochondria interactions. Cell

death and Differentiation. doi: 10.1038/s41418-022-01048-2.

3. Jingru Wang(#), Na Fang(#), Juan Xiong(#), Yuanjiao Du, Yue Cao, Wei-ke Ji* (2021) An ESCRT-dependent

step in fatty acid transfer from lipid droplets to mitochondria through VPS13D−TSG101 interactions. Nature

Communications, 10.1038/s41467-021-21525-5.

4. Yuan Gao(#), Juan Xiong, Qingzhu Chu, Wei-ke Ji* (2021) PDZD8 mediated lipid transfer at ER-LE/lys

contacts is required for LE/lys positioning and neurite outgrowth. Journal of Cell Science, JOCES/2020/255026.

5. Yuanjiao Du(#), Jingru Wang(#), Juan Xiong, Na Fang, and Wei-Ke Ji * (2021) VPS13D interacts with

VCP/p97 and negatively regulates endoplasmic reticulum–mitochondria interactions. Molecular Biology of

the Cell, doi/10.1091/mbc. E21-03-0097.

6. Wei-ke Ji(#), Rajarshi Chakrabarti, Xintao Fan, Lori Schoenfeld, Stefan Strack & Henry N.Higgs* (2017)

Receptor-mediated Drp1 oligomerization on endoplasmic reticulum. Journal of Cell Biology, vol. 216 no.12.

7. Jingru Wang(#), Qingzhu Chu, Weiping Chang, Lin Deng, and Wei-Ke Ji* (2022) A complex containing

RhoBTB3-SHIP164-Vps26B promotes the biogenesis of early endosome buds at Golgi-endosome contacts. BioRxiv. doi: https://doi.org/10.1101/2022.09.30.510409 (under revision at Journal of Cell Biology)

8. Yuanjiao Du(#), Juan Xiong*, Wei-Ke Ji* (2020) Trans-Golgi network-lipid droplet contacts maintain the

TGN integrity and function via lipid transfer activities of VPS13B. BioRxiv. doi: https://doi.org/10.1101/2020.12.16.423147 (under revision at the Contact journal)

9. Wei-ke Ji(#), Anna L. Hatch(#), Ronald A Merrill, Stefan Strack, Henry N. Higgs* (2015) Actin filaments target the maturation of Drp1 oligomers to mitochondrial fission sites. eLife. 2015 Nov 26;4. pii: e11553. doi:

10.7554/eLife.11553.

10. Lili Gong(#), Wei-ke Ji(#), Xiao-Hui Hu(#), Wen-Feng Hu(#), XiangCheng Tang, Zhao-Xia Huang, Ling Li, Mugen Liu, Shihua Xiang, Erxi Wu, Zachary Woodward, Yizhi Liu, Quan Dong Nguyen, and David Wan-Cheng Li* (2014). Sumoylation Differentially Regulates Sp1 to Control Cell Differentiation. Proc Natl Acad Sci USA. 15;111(15):5574-9. doi: 10.1073/pnas.1315034111.

11. Tak Shun Fung, Wei-Ke Ji, Henry N. Higgs*, Rajarshi Chakrabarti* (2019) Two distinct actin filament

populations have effects on mitochondria, with differences in stimuli and assembly factors. Journal of Cell

Science jcs234435 doi: 10.1242

12. Rajarshi Chakrabarti, Wei-Ke Ji, Radu V. Stan, Jaime de Juan Sanz, Timothy A. Ryan, Henry N. Higgs* (2018). INF2-mediated actin polymerization at the ER stimulates mitochondrial calcium uptake, inner membrane

constriction, and division. Journal of Cell Biology, vol. 217 no. 1.

13. AL Hatch, Wei-Ke Ji, RA Merrill, S Strack, HN Higgs* (2016). Actin filaments as dynamic reservoirs for Drp1 recruitment. Molecular Biology of the Cell. 27(20):3109-3121

14. Wei-ke Ji, Xiangcheng Tang, Min Yi, Peichao Chen, Fang-Yuan Liu, Xiao-Hui Hu, Wenfeng Hu, Shujun Fu, Jifang Liu, Kaili Wu, Minxing Wu, Xialin Liu, Lixia Luo, Shan Huang, Zhenzhen Liu, Minbin Yu, Yi-Zhi Liu and David W-C Li* (2013). p53 directly Regulates .A-, and .A3/A1-Crystallin Genes to Modulate Lens

Differentiation. Curr. Mol. Med., 13(6): 968-978.

15. Liu, J(#); Ji, W(#); Sun, S; Zhang, L; Chen, H-G; Mao, Y; Liu, L; Zhang, X; Gong, L; Deng, M; Chen, L; Han, WJ; Chen, P-C; Hu, W-F; Hu, X; Woodward, Z; Liu, W-B; Xiao, Y-M; Liang, S-P; Liu, Y; Liu, S-J; Li, D W-C*; The PP2A-Abeta gene is regulated by multiple transcriptional factors including Ets-1, SP1/SP3, and

RXRalpha/beta., Curr. Mol. Med., 2012, 12(8): 982-994.

16. Chen, P#; Ji, W(#); Liu, F-Y; Tang, H-Z; Fu, S; Zhang, X; Liu, M; Gong, L; Deng, M; Hu, W-F; Hu, X-H; Chen, X-W; Li, Z-L; Li, X; Liu, J; Li, D W-C*; Alpha-crystallins and tumorigenesis., Curr. Mol. Med., 2012, 12(9): 1164-1173.

17. Ji, Weike; Zhao, Zhi; Zhang, Yingzi; Wang, Yu; Ding, Jiuyuan*; Corynebacterium pekinense transketolase:

gene cloning, sequence analysis and expression. Acta Microbiologica Sinica, 2010, 50(11): 1474-1480